Device

Part:BBa_K4407011:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-Nanjing   (2022-09-30)


Pthl-aceE，Expression of aceE with Pthl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1684
    Illegal BglII site found at 2092
    Illegal BamHI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2714
    Illegal AgeI site found at 1425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1956
    Illegal SapI.rc site found at 2076


Design Notes

In our project, we used this device to construct the facultative anaerobic Clostridium tyrobutyricum (C. tyrobutyricum). aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment, therefore allowing engineered C. tyrobutyricum to have an alternative metabolic pathway in the presence of oxygen. This device substitutes the main metabolic enzyme PFOR, which is inactivated under aerobic condition, with PDH in the presence of oxygen. The promotor Pthl starts the transcription of aceE gene and the terminator ends the transcription once it is completed.


Source

BBa_K3443002 + BBa_K103015 + BBa_K4407002 + BBa_K3585002

References